Composite

Part:BBa_K2260001:Design

Designed by: Rachelle Varga   Group: iGEM17_Calgary   (2017-10-25)
Revision as of 07:28, 30 October 2017 by Atika ibrahim (Talk | contribs)

phaC1J4 w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaC1J4 operon was codon optimized for use in E. coli using the . Lastly, histidine tags were added to the operon.

Source

The phaC1 was obtained from Pseudomonas aeruginosa, whereas phaJ4 was obtained from Pseudomonas putida.

References