Composite

Part:BBa_K2260002

Designed by: Rachelle Varga   Group: iGEM17_Calgary   (2017-10-25)
Revision as of 06:47, 27 October 2017 by Samwc98 (Talk | contribs)


Phasin-HlyA

Phasin is an intracellular protein native to PHB-producing bacteria, and binds to PHB deposits within the cell through electrostatic interaction. HlyA is a toxin produced by E. coli that is secreted via an endogenous, single-step type one secretion system. By adding the C-terminus of the HlyA gene to the end of the phasin molecule, the phasin is marked for secretion through the pathway designed for the HlyA. As a result, the phasin molecule binds to intracellular PHB granules and then is secreted due to the HlyA tag, effectively transporting PHB outside of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None