Part:BBa_K2302008

PelB+CALB+His

This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists four basic parts: promoter T7, RBS, pelB and CALB. pelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1023
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 25
  Illegal NgoMIV site found at 51
  Illegal NgoMIV site found at 744
  Illegal NgoMIV site found at 854
  Illegal AgeI site found at 546
  Illegal AgeI site found at 804
  
• 1000
  COMPATIBLE WITH RFC[1000]

result

Electrophoresis of PelB-CALB coding fragment (A) 
Eexpression cassette of pHisx6-Pelb-CALB (B).