Protein_Domain
CALB

Part:BBa_K2302006

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 09:57, 27 October 2017 by Weihongyang (Talk | contribs)


CALB,an enzyme that can hydrolyze fat to fatty acids.

this sequence encodes a functional protein that can hydrolyze fat to fatty acids. The protein is one of the most widely used lipases due to its excellent properties, such as high stereoselectivity and stability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 780
    Illegal AgeI site found at 472
    Illegal AgeI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]

RESULT

To further determine whether the recombinant CALB is functional, we made soft agar containing glyceryl tributyrate on culture plates containing growing bacteria. The result showed that Follower D could secrete degrade glyceryl tributyrate as indicated by transparent ring shaped as “NEFU”, suggesting its production of functional lipase.

NEFU2O17--RESULTS--FIG13.jpg NEFU-China_2017%284%29.png


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Categories
Parameters
None