Project

Part:BBa_K2428000

Designed by: Patricia Zulueta   Group: iGEM17_UChicago   (2017-10-26)
Revision as of 23:21, 26 October 2017 by Pzulueta97 (Talk | contribs)


Mutagenized psB1C3 to create blunt-end restriction sites for cloning not within the prefix/suffix

This part, psB1C3mut, is a circularized form of the plasmid backbone psB1C3. Specifically, the UChicago team cut the linearized psB1C3 sent from iGEM with XbaI and SpeI, then ligated the DNA back together to create a circularized psB1C3 plasmid. QuickChange PCR was performed to create blunt-end restriction sites (such as HpaI) for cloning not within the prefix/suffix region of the original psB1C3 iGEM plasmid. Contains chloramphenicol resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None