Composite
CSP detect

Part:BBa_K2292004

Designed by: THUAN-THIEN TCHEN   Group: iGEM17_CCU_Taiwan   (2017-10-26)
Revision as of 22:38, 1 November 2017 by Loorent (Talk | contribs)


CSP detection through GFP intensity

https://parts.igem.org/Part:BBa_K2292003

Our project aims to detect the risk of caries through three parameters: Competence stimulating peptide, CSP concentration, lactate level, pH value.

K2292003 is the part that we used to detect one of our target molecule, CSP. It will produce membrane receptor, comD. ComD then will bind to CSP. The comD-CSP complex will phosphorylate comE. The phophorylated comE, finally will bind to a comE responsive promoter( on this part) and produce GFP. Through GFP intensity, we will learn the concentration of CSP.

<img src="TCSTS~23~.jpg" style="display:block; margin:auto;">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 786


[edit]
Categories
//function/reporter/fluorescence
Parameters
chassisB. subtilis
functionGFP production through regulation of phosphorylated comE