Part:BBa_K2292005

Lactate detection

Our project aims to detect the risk of caries through three parameters: Competence stimulating peptide, CSP concentration, lactate level, pH value.

This construction is used for lactate quantization We made use of the property of LldR operon. The LldR is transcription factor. Without lactate, it will bind on O1&O2 operator site, form a DNA loop, blocking the transcription. As lactate present, lactate will bind on LldR and open the loop, allowing the transcription to go on and produce GFP. GFP intensity is then detected and deduct lactate level. This construction is cloned into E.coli.

O1 and O2 binding is added on two sides of promoter(J23118)

Sequence:

O1: AATTGGCCCTACCAATT O2: AATTGGCAGTGCCACTT

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 993
  Illegal NheI site found at 1016
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 628
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1691