Regulatory

Part:BBa_K2520023

Designed by: Noa Eden, Dana Kadosh   Group: iGEM17_TECHNION-ISRAEL   (2017-10-25)
Revision as of 12:40, 26 October 2017 by Noa eden (Talk | contribs)


CMV-Tri display-hGH

Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin. It can be used in-vitro and in-vivo to induce ectopic expression of recombinant genes. This promoter is very useful in cells where other promoters, such as CMV, are underactive or silenced (such as stem cells).

Structure

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2264


[edit]
Categories
Parameters
None