Regulatory

Part:BBa_K2247000

Designed by: Jianing Han   Group: iGEM17_Tsinghua   (2017-10-23)
Revision as of 07:22, 26 October 2017 by GBY15 (Talk | contribs)


pGalS promoter of S. cerevisiae

This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//promoter
Parameters
chassisS. cerevisiae
functionGal4-dependent inducible expression
positive_regulatorsyeast Gal4 protein