DNA

Part:BBa_K2429063:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-25)
Revision as of 18:46, 25 October 2017 by Nmyrie (Talk | contribs)

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mKate FF4 Guide Family Member


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The specific motif we’re targeting on the intron is the 3’ splice site. Therefore, the guide sequences tend to target the end of the intron. Furthermore, the guides share a lot of base pairs because they are essentially the same sequences but shifted a certain amount. This practice of tiling guides allows us to make more precise conclusions about what intronic sequences are important for splicing.

Source

The guide sequences were synthesized by IDT.

References