Part:BBa_K2267028
GFP
we Codon optimization Part:BBa_K319039 in G.xylinus
we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are
plux1: https://parts.igem.org/Part:BBa_K2267029
plux3: https://parts.igem.org/Part:BBa_K2267030
plux4: https://parts.igem.org/Part:BBa_K2267031
plux6: https://parts.igem.org/Part:BBa_K2267032
plux8: https://parts.igem.org/Part:BBa_K2267046
listed below. We linked gfp reporter genes luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043
luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041
luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042
luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044
luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045
on them respectively to test the function of modified promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 664
None |