Part:BBa_K2244007
ColE promoter +mhei gene +mcherry gene +T1terminator
The device is a functional plasmid containing a MBC hydrolase gene (mheI) (BBa_K2244004), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry (BBa_J06504), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid.
Biology
ColE promoter(BBa_K2244006) sequence is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.
mCherry(BBa_K2244008) is a red fluorescent protein used as a reporter. It is based on a fluorescent protein that was originally isolated from Discosoma sp.mCherry sequence is codon optimized for E. coli expressionm and can be replaced by any gene sequence in light-regulated studies.
MBC hydrolase gene (mheI)(BBa_K2244004) is derived from carbendazim-degrading strain Mycobacterium sp. SD-4 and is a genomic DNA gene sequence. mheI gene is also evolutionary conserved in many other bacteria strains, such as Nocardioides sp. SG-4G, and Rhodococcus erythropolis.
T1 terminator(BBa_B0010) is the most commonly used terminator.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1250
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1250
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1250
Illegal AgeI site found at 413 - 1000COMPATIBLE WITH RFC[1000]
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