Regulatory
Part:BBa_I766200
Designed by: Nili Sommovilla Group: iGEM07_UCSF (2007-10-22)
pSte2
endogenous promoter of Ste2
Estonia TUIT Contribution
- STE2 promoter (pSTE2) regulates the expression of the STE2 gene coding for the G-protein-coupled receptor for α-factor pheromone. The model for the STE2 gene regulation was proposed by Di Segni et al. in 2011 (Figure 1). According to the model, the STE2 gene is activated in yeast cells by Mcm1. In this case, the canonical poly(A) site in the 3′ UTR is used (long and filled arrow). On the other side, several proteins are involved in the repression of the STE2 promoter in α cells (Mcm1, α2, Ssn6, Tup1, and others).
- The expression of the gene under the STE2 promoter was analyzed in the work of Youk & Lim where they studied yeast cell-to-cell communication (Youk & Lim, 2014). Researchers expressed GFP reporter under pSTE2 promoter and analyzed the correlation of the GFP fluorescence in the far1∆ yeast cells with different α-factor concentrations (Figure 2).
Methodology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
//regulation/negative
//regulation/multiple
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
//regulation/negative
//regulation/multiple
negative_regulators | 1 |
positive_regulators | 2 |