Regulatory

Part:BBa_K2282004

Designed by: Eliott LAFON   Group: iGEM17_IONIS-PARIS   (2017-10-02)
Revision as of 12:46, 25 October 2017 by Bastaaa (Talk | contribs)

Phage lambda promoter regulated by the cI857 repressor

Usage and Biology

Leftward promoter (pL) deriving from phage ƛ is fully repressed at low temperature by the thermolabile cI857 protein. cI857 loss repression activity on pL promoter when temperature rise above 30°C until 42°C when it can no longer bind to pL.

Design consideration

No particular design consideration is to be notified here.

References

Norma A Valdez-Cruz, Luis Caspeta, Néstor O Pérez, Octavio T Ramírez, Mauricio A Trujillo-Roldán - Production of recombinant proteins in E.coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters - Microbial Cell Factories 2010, 9:18.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None