Composite

Part:BBa_K2332053

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 13:48, 25 October 2017 by Paola handal (Talk | contribs)


Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)

We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein upon blue-light (465nm) exposure. For blue-light transcriptional induction, cells must also express EL222 (BBa_K2332004).

To use the GFP-Spytag, one needs to transform E. Coli cells with our Biobrick and expose to blue-light to allow the cells to produce the fusion protein. Then, cells are lysed and the protein purified. It can then be used in combination with our SpyCatcher variants to visualise and quantify their binding affinity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


[edit]
Categories
Parameters
None