Part:BBa_K2505004

atipt4-IVS-IRES-log1-polyA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1193
  Illegal XhoI site found at 2243
  Illegal XhoI site found at 2255
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 739
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 881
  

These two genes(atipt4 and log1) are derived from Arabidopais thaliana and encode enzymes necessary for synthesizing iP (isopentenyladenine) in mammalian cells. iP is a kind of cytokinins that are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. When these genes are introduced to human cells, EA.hy926, the cells produce iP heterologously. The DNA sequences of these genes are optimized for expressing in human cells considering the codon usage. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112: cytokinin synthase) activity and catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, producing cytokinin nucleotides. Note that cytokinin nucleotides are the immature form. LOG1 has the phosphoribohydrolase activity and converts inactive cytokinin nucleotides to the biologically active free-base forms.

Characterization

===Materials & Methods===