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Sample Discontinued
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Composite

Part:BBa_K2244007

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-25)
Revision as of 15:31, 25 October 2017 by Qiucheng (Talk | contribs)


ColE promoter +mhei gene +mcherry gene +T1terminator

The device is a functional plasmid containing a MBC hydrolase gene (mheI) (https://parts.igem.org/Part:BBa_K2244004 BBa_K2244004), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry (https://parts.igem.org/Part:BBa_J06504 BBa_J06504), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid.

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1250
    Illegal AgeI site found at 413
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None