Coding

Part:BBa_K2429029:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)
Revision as of 23:35, 24 October 2017 by Nmyrie (Talk | contribs) (→‎References)


phEF1a Ms2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1474
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1474
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1474
    Illegal BglII site found at 569
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1474
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1474
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Human codon optimized



Source

The part's original source is the RNA bacteriophage. This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.


References

[1]Peabody, D S. “The RNA Binding Site of Bacteriophage MS2 Coat Protein.” The EMBO Journal 12.2 (1993): 595–600. Print. [2]http://what-when-how.com/molecular-biology/qb-replicase-molecular-biology/