Composite

Part:BBa_K2201243

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 19:57, 24 October 2017 by Yanker (Talk | contribs)

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Fusion protein of CFP and YFP with an amber codon in the linker

This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014] and BBa_K2020040 of the [http://2016.igem.org/Team:Aachen/Description#characterization iGEM-team Aachen 2016]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.

Important: This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1420
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None