Composite

Part:BBa_K2201243

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 19:44, 24 October 2017 by Yanker (Talk | contribs)


Fusion protein of CFP and YFP with an amber codon in the linker

This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the iGEM-Team Austin, Texas, 2014. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.

Important: This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1420
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None