Part:BBa_K2201220
CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker
This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker is used to show the properties and uses of the ncAA 2-NPA. The streptavidin of this fusion protein is able to bind biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. This version of the fusion protein is needed to validate the binding and detection properties without the incorporation of the ncAA 2-NPA compared to the part K2201221. Experiments with this protein can deliver the reference values of binding efficiency and fluorescence signal undisturbed by the ncAA. When compared to the values of the amber variant, it can be determined if there is any loss or change in the binding or in the fluorescence properties caused by the 2-NPA or the synthetase.
Important: This part only contains the coding sequence and will not be expressed if transformed. For any further results look up to BBa_K2201320.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 787
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 829
Illegal AgeI site found at 880 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
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