Protein_Domain

Part:BBa_K2319003:Design

Designed by: Raj Magesh Gauthaman   Group: iGEM17_IISc-Bangalore   (2017-10-24)
Revision as of 10:21, 1 November 2017 by Raj Magesh (Talk | contribs) (→‎Design Notes)

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SpyCatcher (optimized for fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 219
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start codon (ATG) was removed from BBa_K1650037 so that SpyCatcher can be used in fusion proteins.

Source

This part was incorporated into our other part BBa_K2319000 using BBa_K1650037 as a template, but with its start codon removed using primers.

References