Part:BBa_K2346005

Positive Feedback Loop for NzFE

This is a device to reduce nitrite ion to its elements: oxygen and nitrogen. The device is regulated by signaling molecule AHL(3OC6HSL). The right p(tetR) promoter will constitutively express Plux’s repressor: LuxR protein. Two LuxR protein molecules can bind with two AHL molecules to form a complex which in turns switches on the PluxR promoter. The down stream LuxL is an enzyme for catalyzing AHL(acyl-homoserine lactones) from normal cell metabolites. The LuxL translated will increase the AHL content in the chassis. This forms a positive feedback loop with increasing AHL resulting in increasing PoPS from PluxR. We utilize this increasing PoPS, and place CDS for NzFE(Nitrite Degradation Fusion Enzyme) in the down stream of the circuit.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4180
  Illegal AgeI site found at 2619
  Illegal AgeI site found at 3991
  Illegal AgeI site found at 5934
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1004