Composite

Part:BBa_K2332041:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-20)
Revision as of 23:32, 23 October 2017 by Paola handal (Talk | contribs) (→‎Source)


Blue light inducible expression of GFP-SpyCatcher (Pblind GFP-SpyCatcher)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


Design Notes

We removed the stop codon in GFP and placed the SpyCatcher sequence after a linker sequence and included a HisTag for protein purification. Pblind promoter was designed by Jayaraman P. et al. (2016)

Source

SpyCatcher sequence was obtained from BBa_K1159200 and GFP from: BBa_E0040. Pblind promoter was designed by Jayaraman P. et al. (2016). The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References