Reporter

Part:BBa_K2429018:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-23)
Revision as of 14:58, 26 October 2017 by Nmyrie (Talk | contribs) (→‎Design Notes)

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3 Exon mKate-HBG Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1762
    Illegal XbaI site found at 1167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1762
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1762
    Illegal XbaI site found at 1167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1762
    Illegal XbaI site found at 1167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 55


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter.

Source

The human beta globin introns came from the genome of HEK cells, and mKate from jellyfish.

References