Regulatory

Part:BBa_K2377014:Design

Designed by: Tuoyu Zhou   Group: iGEM17_Lanzhou   (2017-10-23)
Revision as of 17:14, 27 October 2017 by Freak9631 (Talk | contribs) (→‎Design Notes)


zarp, zinc two-component relulatory system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Improved target: BBa_K1755301（K1755024、K1755305、K1755303）

Improved form We replaced a sensor element（Copper-Responsive Two-Component System BBa_K190017）for the Zinc-Responsive Two-Component regulatory system (contains σ54 promoter).

Mechanism of changed sensor The mechanism of Zinc sensor is related to a intrinsic signaling pathway in Ecoli. Which is known that many sensing proteins ZraS immobilized on the membrane of cell. And when Zn2+ show up in periplasmic space, the ions will combine with ZraS and trigger it autophosphorylation. (ZraS*) The phosphorylation signal will transmit from ZraS* to ZraR, then ZraR* come to ZraR(HydG) binding site[1] that will make σ54promoter start downstream transcription. (ZraS* ,ZraR* means activated condition.) 

     Figure 1   the composition of Zinc-Responsive Two-Component regulatory system

So, we substitute Zinc-Responsive Two-Component regulatory system for the copper one in BBa_K1755301. Successfully construct a new heavy metal sensor.

Source

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References