Translational_Unit

Part:BBa_K2243003

Designed by: Li Yulong   Group: iGEM17_Peking   (2017-10-23)
Revision as of 14:10, 31 October 2017 by Hongchen (Talk | contribs)


D7_Bxb1 gp35

Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.


Usage: D7_Bxb1 gp35 is one of the best expression system. We added RBS D7 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).

Biology: The Bxb1 coding sequence is the part BBa_K22430012.

Design note: We construct this structure by Gibson Assembly. The D7 RBS is generate by random mutation.

Characterize: We characterize the D7_Bxb1 gp35 in a novel structure, which composed with expressional and transcriptional regulatory elements, and with a corresponding reporter system (BBa_K2243006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1105
    Illegal NgoMIV site found at 1192
    Illegal AgeI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


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Parameters
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