Part:BBa_K2244004

mheI

Biology

This part is a functional gene encoding a Carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate, or MBC) pesticide hydrolyzing esterase. mheI encodes MBC-hydrolyzing esterase (MHE) that degrades MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC to 2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa. MBC hydrolase gene (mheI) is derived from carbendazim-degrading strain Mycobacterium sp. SD-4 and is a genomic DNA gene sequence. mheI gene is also evolutionary conserved in many other bacteria strains, such as Nocardioides sp. SG-4G, and Rhodococcus erythropolis.

Usage

A light-repressed gene expression system was used to express MHE in the darkness induction.

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Reference

Xu, J.L., Gu, X.Y., Shen, B., Wang, Z.C., Wang, K. & Li, S.P. 2006. Isolation and characterization of a carbendazim-degrading Rhodococcus sp. djl-6. Curr. Microbiol. 53 (1), 72–76.

Zhang, X.J., Huang, Y. J., Harvey, P.R., Li, H.M. Ren, Y., Li, J.S., Wang, J.N. & Yang, H.T. 2013. Isolation and characterization of carbendazim-degrading rhodococcus erythropolis djl-11. PLoS One 8.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 591
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 250
  
• 1000
  COMPATIBLE WITH RFC[1000]