Composite

Part:BBa_K2505001

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 06:40, 23 October 2017 by TakumaY (Talk | contribs)


pBad/araC-rbs-ahk4

This part produces AHK4, a receptor of iP(isopentenyl adenine: kind of cytokinin). This part was introduced to E.coli(KMI002) and sensed iP synthesized by human cell. The genes(araBAD: PBAD) are transcriptionally regulated by AraC protein. The gene(ahk4) is derived from A.thaliana and optimized for E.coli codon in reference to the codon usage.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1294
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 1542
    Illegal SapI.rc site found at 3177


[edit]
Categories
Parameters
None