Coding

Part:BBa_K2244004

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-22)
Revision as of 05:44, 23 October 2017 by Qiucheng (Talk | contribs)


mheI


This part is a coding gene mheI (for MBC-hydrolyzing enzyme) that encodes MBC-hydrolyzing esterase (MHE) to degrade MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC-2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None