Device
Part:BBa_K2463000:Design
Designed by: Jack Lawrence, Balint Vidos Group: iGEM17_Warwick (2017-10-22)
Biofilm and Motility Regulation Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1806
Illegal BamHI site found at 32
Illegal BamHI site found at 1190
Illegal XhoI site found at 1768 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 105
Design Notes
Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.
Source
Uniprot entries for important proteins:
PLeD Source: [http://www.uniprot.org/uniprot/Q9A5I5 uniprot.org/uniprot/Q9A5I5]
yhjH Source: [http://www.uniprot.org/uniprot/P37646 uniprot.org/uniprot/P37646]
iGEM registry entries regarding parts:
BBa_R0010 - LacI
BBa_B0034 - Ribosome binding site
BBa_K2012002 - PLeD gene (synthesises c-di-GMP)
BBa_C0072 - Mnt repressor
BBa_B1006 - Terminator
BBa_R0073 - Mnt promoter
BBa_K861090 - Yhjh (degrades c-di-GMP)
BBa_B1003 - Second Terminator