Coding

Part:BBa_K2272003

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)
Revision as of 05:37, 1 November 2017 by Stevenscherping (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cyt(Cytochrome)

This sequence codes for cytochrome found in Nitrosomonas europaea that catalyze the conversion of hydroxylamine (NH2OH) quantitatively to N2O under anaerobic conditions by transporting electrons from HAO to AMO, the other two main enzymes involved in the nitrification circuit. By incorporating P. denitrificans TAT sequence within this coding sequence, we expect that the cytochrome protein will be directed to the periplasm of the cell. For further characterization information, refer to our complete device: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2272020

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 49
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 118
    Illegal NgoMIV site found at 852
    Illegal NgoMIV site found at 924
    Illegal NgoMIV site found at 972
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1499
    Illegal BsaI.rc site found at 1655


[edit]
Categories
Parameters
None