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Part:BBa_K2407107:Design

Designed by: Yan Zheng   Group: iGEM17_Tianjin   (2017-10-17)
Revision as of 04:56, 25 October 2017 by Tjuyoung (Talk | contribs) (→‎Design Notes)


HO gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
    Illegal BglII site found at 954
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1290


Design Notes

Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p.

Source

We use PCR to amplify the part from prs416 carotene plasmid.

References

McAlister L and Holland MJ (1985) Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15013-8 PMID: 2999100 McAlister L and Holland MJ (1985) Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15019-27 PMID: 3905788 Shen H, et al. (2014) Structural insights into RNA recognition properties of glyceraldehyde-3-phosphate dehydrogenase 3 from Saccharomyces cerevisiae. IUBMB Life 66(9):631-8 PMID: 25288528