Reporter

Part:BBa_K2440008:Design

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 12:23, 30 October 2017 by Jhyzxqj (Talk | contribs) (→‎References)


Fluc (with BsmBI restriction site)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1314


Design Notes

None.


Source

We obtain it from PCR.

References

[1] Fraga H, Fernandes D, Novotny J, Fontes R, Esteves da Silva JC. (Jun 2006). "Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation". Chembiochem.7(6):929-35. PMID: 16642538 DOI: 10.1002/cbic.200500443

[2] Heise K, Oppermann H, Meixensberger J, Gebhardt R, Gaunitz F. (May 2013)."Dual luciferase assay for secreted luciferases based on Gaussia and NanoLuc". Assay Drug Dev Technol.11(4):244-52. PMID: 23679848 DOI: 10.1089/adt.2013.509