Part:BBa_K2415003

MTX1

Mtx 1 was found in Bacillus sphaericus(Bs), an aerobic, Gram-positive, spore-forming bacterium, and it is pathogenic to mosquitoe larvae. Mosquitocidal strains of B. sphaericus can be divided into two groups based on their toxicity to mosquitoe larvae .

Three MTX proteins, MTX1, MTX2 and MTX3, were firstly isolated from the low toxicity strains but have also been found in some high-toxicity strains. They are produced in the vegetative phase of growth and are completely degraded in sporulation. MTX has a high toxic level against Culex quinquefasciatus (LC50 ~15 ng ml-1) and Aedes aegypti (LC50 ~50 ng ml-1) larvae.

The mosquitocidal toxin (MTX) from Bacillus sphaericus(Bs) is a member of ADP ribosyltransferases family . Mtx1 is a ~100 kDa soluble protein, consisting of two parts including the N-terminal 27 kDa domain containing ADP-ribosylase activity and the C-terminal 70 kDa domain which is very similar to the carbohydrate-binding protein. The mature MTX (without the putative signal sequence) is processed into a 27 kDa N-terminal fragment and a 70 kDa C-terminal fragment by trypsin. The N-terminal ADP ribosyl moiety connects to protein amino acid residues in the intestinal cells of mosquito larvae, and thus the Mtx1 disable the proteins and kill the cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1970
  Illegal BamHI site found at 2009
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 506
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1269