Regulatory
pleP

Part:BBa_K2374001:Design

Designed by: Qian Zeng   Group: iGEM17_Tongji_China   (2017-10-20)
Revision as of 02:52, 30 October 2017 by Zjeng (Talk | contribs) (→‎Design Notes)


TH (ple) promoter-> (fruit fly)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We get this 452bp sequence from FlyBase ([http://flybase.org/reports/FBgn0005626.html] Dmel\ple)


site direct mutangenesis: EcoR I (184) GAATTC->GATTTC Xba I (219) TCTAGA->TGTAGA

Source

NT_037436.4 (NCBI)

References