Part:BBa_K2328050

GLBP + Linker.a

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 771
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 88
  Illegal NgoMIV site found at 610
  Illegal NgoMIV site found at 1050
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 274
  Illegal BsaI.rc site found at 691
  

Usage

The GNB/LNB substrate-binding membrane protein (GL-BP) is a membrane protein belonging to the ATP binding cassette (ABC) protein family, Which transports lacto-N-biose (i.e.,N-acetyl-3-O-([3-D-galactopyranosyl)D-glucosamine) and galacto-N-biose (i.e.,N-acetyl-3-O-([3D-galactopyranosyl)-D-galactosamine) of bifidobacterium. ABC proteins are important membrane proteins that actively transport specific substances on the cell membranes of all organisms using an energy called adenosine triphosphate (ATP), and various ABC proteins are present on the cell membranes. Therefore, if an appropriate promoter is used, GL-BP, which is an ABC protein, is ubiquitously expressed in bacteria belonging to the genus bifidobacterium, which have a cellular function for expressing GL-BP on the surface thereof. Linker.a is used to separate GL-BP from other core parts.

Biology

Bifidobacterium longum is a strictly anaerobic bacterium and there’s no oxygen in anaerobic bacteria, so the co- expression system won’t work, we choose the system of surface display. Our concept is proposed that the smURFP should be fused with GL-BP and displayed on the surface of a microbial cell so that they can be combined with BV.

Reference

[1] Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, et al. (2010) Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model. Vaccine 28: 6684–6691.