Part:BBa_K2286003

EchA: Epichlorohydrin epoxide hydrolase

EchA is an epoxide hydrolase derived from Agrobacterium radiobacterAD1, which can undergo the ring-opening reaction of various epoxides. It is reported that EchA Shows better activity for Epoxohydrin, Epibromohydrin and Epifluorohydri in various epoxides[1]。 Since this enzyme is derived from prokaryotic cells, the original sequence is suitable for expression in prokaryotic cells, and this sequence is codon optimized for petunia system and is more suitable for plant system expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Molecular weight

This epoxide hydrolase gene codes for a protein of 294 amino acids with a molecular mass of 35 kDa[2].

Effect of pH on enzyme activity

It is reported that the activity of the oxidative hydrolase derived from Pseudomonas sp. Strain AD1 was greatly affected by the pH[2]. Therefore, we tested the activity of EchA expressed by Escherichia coli MC1061 at different pH levels. The results showed that the best activity of EchA located about pH 8.5 in the 50 mM Tris / sulphate buffer at 37℃. This is consistent with the results reported in the literature[2].

Fig 1.Effect of pH on enzyme activity.