Coding

Part:BBa_K2287027

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-20)
Revision as of 14:22, 20 October 2017 by Fred hjy (Talk | contribs)


purF K326Q

It is the coding sequence of the K326Q mutant of glutamine phosphoribosylpyrophosphate amidotransferase that catalyzes the transformation from PRPP to PRA in de novo purine biosynthesis. We introduced a mutation K326Q to the enzyme based on "Identification of Sites for Feedback Regulation of Glutamine 5-Phosphoribosylpyrophosphate Amidotransferase by Nucleotides and Relationship to Residues Important for Catalysis"(Gaochao Zhou et al. ,1993) to remit the feedback inhibition by GMP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 876
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None