Part:BBa_K2387003
eYFP controlled by inducible araC/pBAD promoter
Fluorescent protein eYFP is put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.
It is used as to control the functioning of inducible promoter araC/pBAD.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Functional Parameters
In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([ https://parts.igem.org/Part:BBa_K2387032 BBa_K2387032]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.
Table 1: Split Proteins. | ||
---|---|---|
Protein | Part Number (Full length) | Part Number (Split Protein) |
mRFP | BBa_K2387054 | BBa_K2387055 |
eYFP | BBa_K2387003 | BBa_K2387065 |
mVenus | BBa_K2387045 | BBa_K2387046 |
sfGFP | BBa_K2387047 | BBa_K2387048 |
mCerulean | BBa_K2387052 | BBa_K2387053 |
None |