RNA

Part:BBa_K2206011:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-19)
Revision as of 23:47, 19 October 2017 by SLovat (Talk | contribs) (→‎Design Notes)

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Anti-miRNA for hsa-miR-27b-3p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The hybridisation domain for hsa-miR-27b-3p must be perfectly complementary to the 3' end of hsa-miR-27b-3p.

Outside of the microRNA hybridisation domain, the anti-miRNA has no sequence constraints.

Source

This part is a synthetic toehold switch that was designed in silico.

References