Part:BBa_K2206010
Toehold switch for hsa-miR-27b-3p (second series)
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch
Bba_X and hsa-miR-15b-5p contain roughly half of the trigger sequence each. Colocalization of the split trigger RNA sequences results in a functional trigger RNA that is capable of activating the toehold switch. This part codes for a toehold switch that contains a region that is complementary to the complete trigger RNA sequence formed of Bba_X and hsa-miR-15b-5p Therefore, this part can be used to regulate the expression of any protein in response to presence of both Bba_X and hsa-miR-15b-5p.
We found that the transcripts produced by our other toehold switch parts were toxic to E.coli. We therefore recommend using this part with a non-leaky promoter. For reference, we found the part BBa_K808000 to be too leaky.
This part contains a strong RBS sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 65
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