Part:BBa_K2206011
Anti-miRNA for hsa-miR-27b-3p
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
The trigger sequence can be split into different RNA molecules. Colocalization of the RNA molecules containing the split trigger parts results in a complete trigger RNA sequence that is capable of activating the toehold switch.
The trigger RNA sequence for BBa_K2206010 is divided among hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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