Coding

Part:BBa_K2387059:Design

Designed by: Linda van Oosten   Group: iGEM17_Wageningen_UR   (2017-10-19)
Revision as of 13:58, 19 October 2017 by LindavanOosten (Talk | contribs) (→‎Design Notes)

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Extracellular domain of ISG65 of T.b. gambiense


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed illegal PstI restriction site by silent mutation (GCA GAA --> GCC GAA) at Ala356

Source

PCR amplified from genomic DNA of Trypanosoma brucei gambiense

References