Composite
Part:BBa_K2443031
Designed by: Lethbridge iGEM 2017 Group: iGEM17_Lethbridge (2017-10-18)
RF3 optimized for expression in E. coli
Release factor 3 (RF3) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF3 has been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 919
Illegal AgeI site found at 980 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1143
[edit]
Categories
Parameters
//collections/cell-free_tx-tl
None |