Regulatory

Part:BBa_K2206010:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-18)
Revision as of 23:24, 19 October 2017 by SLovat (Talk | contribs) (→‎Design Notes)

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Toehold switch for hsa-miR-27b-3p (second series)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 65


Design Notes

There must be no stop codons between the start codon and the protein coding sequence.

The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more details about design principles visit: http://2017.igem.org/Team:CLSB-UK/Design

Source

This part is a synthetic toehold switch that was designed in silico.

References