Device

Part:BBa_K2325102

Designed by: Huixian Li   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-18)
Revision as of 15:46, 27 October 2017 by Tamce (Talk | contribs)


Phosphite dehydrogenase expression device

The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.

Usage and Biology

BBa K2325102--1.png
Figure 1. Growth curve of E.coli BL21(DE3) and the engineering E.coli BL21(DE3)(pGEX-ptx) expressing phosphite dehydrogenase in a medium contains Na2HPO3•5H20 (1.32mM) and NH4Cl serving as the phosphorus and nitrogen sources, respectively.

The E.coli expressing phosphite dehydrogenase outcompeted a control strain in phosphite-containing media after 72 hours.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None