Part:BBa_K2387047

sfGFP controlled by inducible araC/pBAD promoter

sfGFP is a bright green monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. sfGFP presents a maximum pick of excitation at 485 nm and a maximum pick of emission at 510. sfGFP was engineered from GFP to achieve a faster maturation and a higher stability.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  Illegal SapI.rc site found at 1251
  

Functional Parameters

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).

Figure 1: Absorbance and fluorescence spectra of sfGFP, measured after induction with 0.002% arabinose.

This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system (BBa_K2387032). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.

Table 1: Split Proteins.
Protein	Part Number (Full length)	Part Number (Split Protein)
mRFP	BBa_K2387054	BBa_K2387055
eYFP	BBa_K2387003	BBa_K2387065
mVenus	BBa_K2387045	BBa_K2387046
sfGFP	BBa_K2387047	BBa_K2387048
mCerulean	BBa_K2387052	BBa_K2387053