Coding

Part:BBa_K2232003

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-17)
Revision as of 07:15, 28 October 2017 by James zhang (Talk | contribs)


C125-TupA

Gene tupA was cloned from the chromosomal DNA of facultative alkaliphilic strain Bacillus lentus C-125. The primary translation product of this gene, TupA, is likely a cytoplasmic protein(57.3 kDa) consisting of 489 amino acid residues.It was demonstrated that TupA is involved in the synthesis of TUP,a copolymer of polyglutamic acid (PGlu) and polyglucuronic acid (PGlcU),which is one of major structural components in the cell wall of the Bacillus lentus C-125 and can neutralize the extracellular hydroxyl. A mutant defective in TUP synthesis grows slowly at alkaline pH,indicating this gene plays a key role in pH homeostasis and increases the alkali resistance of Bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 789
    Illegal BsaI.rc site found at 1181


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Categories
Parameters
None