Composite

Part:BBa_K2505008:Design

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 14:09, 1 November 2017 by TakumaY (Talk | contribs)


Ptet-rbs-traR-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The traR gene is derived from A. Tumefaciens and the codon of this part is optimized for E. coli in terms of codon usage. TraR protein recieves Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), leading to the activaion of the trascription.

Source

This part is derived from Agrobacterium.

References

Conserved cis-Acting Promoter Elements Are Required for Density-Dependent Transcription of Agrobacterium tumefaciens Conjugal Transfer Genes, 1995 CLAY FUQUA et.al